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The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI. Lambda DNA is digested by the Eco RI endonuclease. In this experiment, students will use gel electrophoresis to analyze the digestion products. This complete activity kit includes Lambda DNA, Dryzymes®, reconstitution buffer, restriction enzyme reaction buffer, enzyme-grade water, standard DNA Fragments, agarose powder, and FlashBlue™ Stain. Coupon is included to redeem all perishable materials at your convenience. Requires the use of electrophoresis apparatus, power supply, automatic pipette with tips, waterbath, balance, microwave or hot plate, white light source, lab glassware, pipette pumps or bulbs, metric rulers, floating racks, distilled or deionized water, and ice. Materials provided for ten restriction digests and 5 agarose gels. Time requirements: 90 minutes.
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